dapi (Vector Laboratories)
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Vector Laboratories
dapi
Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 3097 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mounting+medium+containing+dapi/VECTASHIELD+Hardset+Antifade+Mounting+Medium+with+DAPI/pm42757461-74-5-9
Average 96 stars, based on 3097 article reviews
Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 3097 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mounting+medium+containing+dapi/VECTASHIELD+Hardset+Antifade+Mounting+Medium+with+DAPI/pm42757461-74-5-9
Average 96 stars, based on 3097 article reviews
dapi - by Bioz Stars,
2026-10
96/100 stars
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Staining:Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization Article Snippet: .. Subsequently, slides were stained with antibodies against NPM1 (ab10530, Abcam; 1:1000) and HP1α (ab109028, Abcam; 1:1000), then mounted with VECTASHIELD containing Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways Article Snippet: .. Thereafter, sections were stained with a recombinant rabbit monoclonal antibody against Nlrp3 (invitrogen, Cat#: MA5-32255, RRID: AB_2809541 ), Casp1 p20 (cleaved Asp296) polyclonal antibody (invitrogen, Cat#: PA5-99390, RRID: AB_2818323 ), and Nek7 polyclonal antibody (invitrogen, Cat#: PA5-101861, RRID: AB_2851293 ) at 1:100 dilution in 1.5% horse serum in PBS, while NF-κB p65 was incubated with mouse monoclonal antibody (SantaCruz, Cat#: A-12 sc: 514451, RRID: AB_2891257 ) at 1:25 dilution overnight at 4 °C followed by secondary antibody [goat anti-rabbit IgG Alexa Flour-488 (Nlrp3 and Nek7) and 594 (Casp1 and NF-κB p65)] at 1:1000 dilution in PBS for 2 h. Sections were then washed 3 times with PBS and counterstained with Article Title: MMRi62 induces iron depletion–driven apoptosis through a ferritin-independent mechanism Article Snippet: Coverslips were subsequently washed three times with PBS and incubated with fluorescently labelled phalloidin (1:500; Cytoskeleton, PHDH1-A) for 1 h at room temperature to visualize the actin cytoskeleton. .. Following three additional washes with PBS, coverslips were mounted onto microscope slides using mounting medium containing Article Title: Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and applications Article Snippet: After quick freezing, the CEO gel was cryo-sectioned at a thickness of 8 μm using a freezing microtome (Epredia HM 525 NX Cryostat Package, Epredia, MI, USA) and stained with Brilliant Blue R Staining Solution (B6529, Sigma-Aldrich) for 10 min at RT to visualize total proteins. .. After several washes, the samples were incubated with secondary antibodies for 1 h at RT and then mounted with Vectashield® mounting medium containing 4, Recombinant:Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways Article Snippet: .. Thereafter, sections were stained with a recombinant rabbit monoclonal antibody against Nlrp3 (invitrogen, Cat#: MA5-32255, RRID: AB_2809541 ), Casp1 p20 (cleaved Asp296) polyclonal antibody (invitrogen, Cat#: PA5-99390, RRID: AB_2818323 ), and Nek7 polyclonal antibody (invitrogen, Cat#: PA5-101861, RRID: AB_2851293 ) at 1:100 dilution in 1.5% horse serum in PBS, while NF-κB p65 was incubated with mouse monoclonal antibody (SantaCruz, Cat#: A-12 sc: 514451, RRID: AB_2891257 ) at 1:25 dilution overnight at 4 °C followed by secondary antibody [goat anti-rabbit IgG Alexa Flour-488 (Nlrp3 and Nek7) and 594 (Casp1 and NF-κB p65)] at 1:1000 dilution in PBS for 2 h. Sections were then washed 3 times with PBS and counterstained with Incubation:Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways Article Snippet: .. Thereafter, sections were stained with a recombinant rabbit monoclonal antibody against Nlrp3 (invitrogen, Cat#: MA5-32255, RRID: AB_2809541 ), Casp1 p20 (cleaved Asp296) polyclonal antibody (invitrogen, Cat#: PA5-99390, RRID: AB_2818323 ), and Nek7 polyclonal antibody (invitrogen, Cat#: PA5-101861, RRID: AB_2851293 ) at 1:100 dilution in 1.5% horse serum in PBS, while NF-κB p65 was incubated with mouse monoclonal antibody (SantaCruz, Cat#: A-12 sc: 514451, RRID: AB_2891257 ) at 1:25 dilution overnight at 4 °C followed by secondary antibody [goat anti-rabbit IgG Alexa Flour-488 (Nlrp3 and Nek7) and 594 (Casp1 and NF-κB p65)] at 1:1000 dilution in PBS for 2 h. Sections were then washed 3 times with PBS and counterstained with Article Title: Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and applications Article Snippet: After quick freezing, the CEO gel was cryo-sectioned at a thickness of 8 μm using a freezing microtome (Epredia HM 525 NX Cryostat Package, Epredia, MI, USA) and stained with Brilliant Blue R Staining Solution (B6529, Sigma-Aldrich) for 10 min at RT to visualize total proteins. .. After several washes, the samples were incubated with secondary antibodies for 1 h at RT and then mounted with Vectashield® mounting medium containing 4, Microscopy:Article Title: MMRi62 induces iron depletion–driven apoptosis through a ferritin-independent mechanism Article Snippet: Coverslips were subsequently washed three times with PBS and incubated with fluorescently labelled phalloidin (1:500; Cytoskeleton, PHDH1-A) for 1 h at room temperature to visualize the actin cytoskeleton. .. Following three additional washes with PBS, coverslips were mounted onto microscope slides using mounting medium containing |